021-57609976
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一、细胞基本属性
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细胞名称
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DMS-114细胞
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细胞别称
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DMS-114; DMS114; Darmouth Medical School 114
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商品货号
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ORC1124
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种属来源
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人
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年龄性别
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男性；68岁
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组织来源
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肺
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生长特性
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贴壁细胞
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细胞形态
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上皮细胞样
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背景简介
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The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy.The patient had not received prior therapy. The cells express HLA class I and class II antigens. Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin.
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生物安全等级
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BSL-1
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细胞规格
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1×106cells/T25培养瓶或者1mL冻存管包装
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支原体检测
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无
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基因表达情况
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adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP)
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培养基
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Waymouth'sMB 752/1 或者 1640 基础培养基；优质胎牛血清，10%；双抗，1%
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培养条件
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气相：空气，95%；CO2，5%, 温度：37℃
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冻存条件
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冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮
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