一、细胞基本属性
|
|
细胞名称
|
THLE-2细胞
|
细胞别称
|
THLE2; Transformed Human Liver Epithelial-2
|
商品货号
|
ORC1056
|
种属来源
|
人
|
年龄性别
|
男性
|
组织来源
|
肝脏;左叶
|
生长特性
|
贴壁细胞
|
细胞形态
|
上皮细胞样
|
背景简介
|
THLE-2 is an epithelial cell that was isolated from the left lobe of a donor.THLE-2 and THLE-3 cells express phenotypic characteristics of normal adult liver epithelial cells. They are nontumorigenic when injected into athymic nude mice, have near-diploid karyotypes, and do not express alpha-fetoprotein. [RF84750] THLE-2 and THLE-3 cells metabolize benzo[a]pyrene, N-nitrosodimethylamine, and aflatoxin B1 to their ultimate carcinogenic metabolites that adduct DNA, which indicates functional cytochrome P450 pathways.
|
生物安全等级
|
BSL-2
|
细胞规格
|
1×106cells/T25培养瓶或者1mL冻存管包装
|
支原体检测
|
无
|
基因表达情况
|
|
培养基
|
上皮细胞专用培养基
|
培养条件
|
气相:空气,95%;CO2,5%, 温度:37℃
|
冻存条件
|
冻存液:55%DM/F12+40%FBS+5%DMSO 温度:液氮
|